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Testing Labs 

   In order to have the best possible results in your peptide research it is crucial to have quality product. The only way to guarantee the purity and integrity of a research peptide is by having it analyzed in a reputable lab facility.

    A purity of 98% and above is recommended for research peptides, 99% purity being optimal.

     Researches must request and familiarize themselves with Certificates of Analysis (COA) which provide insight into the peptide composition.

Labs test for purity and quality.

Frequently Used Labs 

1. Janoshik
2. Chromate
3. Forever Young Pharmacy
4. MZ Biolabs 

5. Pharmetric Lab
6. Vanguard Lab 

7. DNA Testing

8. Krause Labs

9. Bio Regen 

10. SR Biolabs

11. Prime Analytical Labs 

12. Veritas Analytic

13.Trust Pointe Analytics

14. Peptide Test 

General Information 

Peptide Purity Chart Crude (preferably higher purity)

   - Fist pass non-sensitive screening

   - Mutation screening

   - Sequence optimization

   - Protein-protein interaction

   - Receptor-ligand interactions

 

Immuno Grade Purity > 75%

   - ELISA testing - Peptide arrays

   - Antigens for polyclonal antibody production or affinity purification

 

Biochemistry Grade >85%

   - In-vitro bioassays

   - Epitope mapping

   - Polyclonal antibody production

   - Phosphorylation studies

   - Cell attachment studies

   - Protein electrophoresis applications

   - Semi-quantitative enzyme-substrate studies

   - Peptide blocking studies for western blot (non-quantitative)

   - Coupling to chromatography resins for affinity purifications

 

High purity grade >95%

   - In-vitro bioassays

   - NMR studies

   - Quantitative receptor

   - Ligand interactions studies

   - Quantitative blocking and competitive inhibition studies

   - Quantitative phosphorylation studies

   - Quantitative proteolysis studies

 

Clinical Grade Purity >98 %

   - Crystallography

   - Cosmetic peptides for cosmeceuticals industry

   - Clinical trials

   - SAR studies

Here are some reasons testing peptide formulations is important:

  • Identification: Tests confirm that the correct peptide is present in the formulation.

  • Purity Testing: Several techniques can detect impurities, contaminants, or unwanted byproducts.

  • Potency (Assay): The test measures the exact concentration of the active peptide.

  • Stability Testing: Heat, light, and other environmental factors can make peptides go bad. Stability tests check how peptide-based formulations hold up under different conditions to help set the drug’s shelf life.

 

Peptide purity is tested using a combination of analytical methods, primary reversed-phase high-performance liquid chromatography (RP-HPLC) and Mass Spectrometry (MS). HPLC separates the peptide from impurities, while MS confirms its identity and molecular weight. Other techniques, like amino acid analysis (AAA) and nuclear magnetic resonance (NMR), are also used to verify the peptide's composition and structure.  

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